Translational_Unit

Part:BBa_J18939:Design

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-26)


FKBP ~ bla-frag2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 456
    Illegal AgeI site found at 732
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 589
    Illegal BsaI.rc site found at 20


Design Notes

Constructs were assembled according to R.F.C. 25.

All parts were codon optimized for E. coli.


Source

synthetic

References